thr77 login Options
thr77 login Options
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become noticed in many tumors, which leads to chromosome instability, and thus may possibly lead to tumorigenesis. This protein was identified to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a vital purpose in endorsing cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are explained. Two extra splice variants were being noted but in depth nucleotide sequence data is just not but available.
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Blocking peptides are peptides that bind specifically on the target antibody and block antibody binding. These peptide normally incorporates the epitope acknowledged by the antibody.
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This guide outlines further more doable optimization of mobile and immunoassay parameters to be sure the very best final results are...
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This information outlines further attainable optimization of mobile and immunoassay parameters to make sure the very best results are...
Antibodies certain to the blocking peptide now not bind towards the epitope over the goal protein. By evaluating the staining from the blocked antibody compared to the antibody get more info by itself, one can see which staining is particular.
Goal Data Cyclin E belongs to your hugely conserved cyclin relatives, whose customers are characterized by a spectacular periodicity in protein abundance throughout the mobile cycle. Cyclins operate as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a fancy with and features like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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